Diagnosis of Fanconi's anemia by nitrogen mustard induction of chromosome breakage in fibroblasts
Desangles, F.
Pathologie-Biologie 39(2): 99-104
1991
ISSN/ISBN: 0369-8114 PMID: 2017342 Document Number: 377235
Induction by nitrogen mustard of chromosome breakage in fibroblasts is proposed as an antenatal test for the diagnosis of Fanconi syndrome. After trypsinization of fibroblasts, addition to the culture medium of 0.025 micrograms/ml nitrogen mustard significantly increased chromosomal breaks in cells from patients with Fanconi syndrome but not in cells from controls. This protocol may prove useful for the antenatal diagnosis of Fanconi syndrome.