Human red blood cells as bioreactors for the inactivation of harmful xenobiotics
Fazi, A.; Mancini, U.; Piatti, E.; Accorsi, A.; Magnani, M.
Biotechnology and Applied Biochemistry 14(1): 60-68
1991
ISSN/ISBN: 0885-4513 PMID: 1910585 Document Number: 376268
Human red blood cells are able to inactivate lipophilic electrophiles by conjugation with reduced glutathione. This metabolic ability was found to be limited by the rate of permeation of the xenobiotic into erythrocytes and by the amount of available reduced glutathione. By a procedure of hypotonic dialysis, isotonic resealing and reannealing human red blood cells were overloaded with increasing amounts of reduced glutathione up to three- to fourfold the normal level without modification of their metabolic functions or of their energetic state. These over-loaded erythrocytes were able to conjugate increasing amounts of xenobiotics and to export the resulting conjugates from the cells. These properties of glutathione overloaded erythrocytes are significant for the use of carrier erythrocytes in cases of acute intoxication by lipophilic electrophiles.