Changes in the membrane potential, the cytoplasmic Ca2+ concentration and in the transport of fluorescein anions across the macrophage plasma membrane under the influence of O2 (-) and H2O2 in small concentrations
Gamaleĭ, I.A.; Kaulin, A.B.; Kirpichnikova, K.M.
Tsitologiia 33(7): 79-85
1991
ISSN/ISBN: 0041-3771 PMID: 1668548 Document Number: 376129
It has been shown that under the influence of superoxide anion (300 nM) and hydrogen peroxide (100 nM) on murine peritoneal macrophages, the depolarization of membrane takes place, and a change in the rate of fluorescein anion efflux from the cells occurs. Hydrogen peroxide (but not superoxide anions) causes a transient increase in the cytoplasmic Ca2+ concentration. These changes are regarded as early signs of macrophage activation. It is assumed that macrophage activation with reactive oxygen intermediates at such a low concentration might be interpreted as follows: a small portion of stimulated phagocytes activates the whole population with the aid of the products of their own oxidative burst.