The phenotype of Arabidopsis thaliana det1 mutants suggests a role for cytokinins in greening
Chory, J.; Aguilar, N.; Peto, C.A.
Symposia of the Society for Experimental Biology 45: 21-29
1991
ISSN/ISBN: 0081-1386 PMID: 1843409 Document Number: 374995
When grown in the dark, these mutants develop features of light-grown plants as determined by morphological, cellular and molecular criteria. In light-grown plants, mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and the chloroplast developmental programme. Addition of 2-isopentenyladenine (2iP), kinetin or benzyladenine to the growth medium of dark-germinated wild-type seedlings resulted in seedlings that resembled det1 mutants instead of having the normal etiolated morphology. Like det1 mutants, these dark-grown seedlings now contained chloroplasts and had high levels of expression for genes that are normally light regulated. The results, it is thought, may implicate abnormal cytokinin levels or increased sensitivity to cytokinins in explaining the observed phenotypes of det1 mutants.