Enzyme histochemical expressions of smooth muscle cell modulation in arterial development, hypertension and remodeling
Hadjiisky, P.; Bourdillon, M.C.; Grosgogeat, Y.
Cellular and Molecular Biology 37(5): 531-540
1991
ISSN/ISBN: 0145-5680 PMID: 1934022 Document Number: 373770
In order to define metabolic profiles of smooth muscle cell (SMC) modulation, 16 enzyme activities linked to nucleotide hydrolysis, lipolysis, lysosomal reactivity and intermediate glucose catabolism were compared in four rat arterial models, exhibiting four metabolic phenotypes of modulated smooth muscle cells: (i) "primary synthetic" state in immature aorta; (ii) "contractile" state in adult aorta; (iii) "hypertensive" state in aorta of hypertensive rat, SHR; (iiii) "secondary synthetic" state in diffuse intimal thickening of ligated carotid artery. Contractile SMC presented strong activities of enzymes linked to nucleotide ester hydrolysis and contractility (ATP-A-Ca, ATP-A-Mg, ATP-A-Ca/Mg, 5'nucleotidase) and to lipolytic process (butyryl cholinesterase, acid esterase). These enzyme activities were more pronounced in "hypertensive SMC". In contrast, the same enzymes were weakly active or not expressed in "synthetic SMC". Increased lyososomal enzyme reactivity was a particular expression of "secondary synthetic SMC". The observed enzyme abnormalities in actively modulated SMC(proliferative-synthetic phenotype) might be related to the loss of contractility and to the enhanced cell proliferation and lipid accumulation, characteristic features of modulated SMC in atherogenesis.