The composition of a population of cloned cultures of revertant Vibrio cholerae L forms

Danilkina, E.B.; Lomov, I.M.; Kolesnikov, N.N.; Lobanov, V.V.; Vlasov, V.P.; Cherkasova, L.R.; Badalova, I.M.

Mikrobiolohichnyi Zhurnal 52(3): 10-16


ISSN/ISBN: 0201-8462
PMID: 2215283
Document Number: 366882
The process of L-transformation and L-transformed state duration have been studied for their effect on variability of main characters of revertant cultures of choleric vibrions L-forms at the population level with the use of cloned cultures of the choleric vibrions. The study was conducted on two strains of the choleric vibrion of the eltor biovar in different periods of storage in the L-transformed state (1, 3, 6 months). It has been revealed that characters of the species and biovar remained stable despite the influence of L-transforming agents. The characters of clone cultures characterizing virulence (sensitivity to KhDF phages, hemolytic activity, toxin production and virulence for sucking rabbits proved to be subjected to variability to the greatest extent with simultaneous preservation of the toxin-production gene. A resistant change of the serovar (from Inaba to Ogava) is observed only in one revertant-subculture of the virulent strain.

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