Production and application of monoclonal antibody specific for human chorionic gonadotropin

Stamenova, M.; Kehayov, I.; Kyurkchiev, S.; Despodova, T.

Folia Biologica 36(2): 81-90

1990


ISSN/ISBN: 0015-5500
PMID: 2192923
Document Number: 366308
Spleen cells from mice immunized with human chorionic gonadotropin (hCG) were fused with mouse myeloma cells and four hybridomas, secreting monoclonal antibodies, were selected for further studies. In cross-check tests against tissue extracts and peptide hormones it was established that mAb 1B10 (IgG1) reacted positively against hCG only but not with other hormones. Ascites from this hybridoma was fractionated by ammonium sulphate precipitation and by FPLC to isolate the IgG fraction. Subsequently, the purified mAb was conjugated with horseradish peroxidase and used for development of a simple test for pregnancy diagnosis. Some preliminary experiments have shown that the test is very specific and reproducible.

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