The p53 nuclear localisation signal is structurally linked to a p34cdc2 kinase motif
Addison, C.; Jenkins, J.R.; Stürzbecher, H.W.
Oncogene 5(3): 423-426
1990
ISSN/ISBN: 0950-9232 PMID: 2156209 Document Number: 363859
We have identified a region of human p53 proteinwith striking homology to a sequence motif on Simian Virus 40 T antigen which includes the nuclear localisation signal. Mutation of basic amino acid residues in this region of p53 (residues 312 to 323; SSSPQPKKKP) compromises transport of p53 protein to the nucleus. The sequence functions efficiently as a nuclear localisation signal when fused to Escherichia coli .beta. galactosidase. Serine 315 within this p53 structural motif is phosphorylated in vitro by the cell cycle kinase p34cdc2. Thus in both T antigen and p53, nuclear localisation signal and p34cdc2 kinase acceptor residue map to a contiguous region of primary amino acid sequence.