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Trial application of transfection tests in feasibility studies for transforming DNA in white blood cells of patients with chronic lymphatic leukemia

Jagla, K.; Mucha, B.; Wilemska, A.; Marcinkowska, A.; Soszyńska, K.; Gołuszko, E.; Kremplewska, E.; Szadziewska, D.; Domaniewski, J.

Polski Tygodnik Lekarski 45(21-22): 412-416

1990

ISSN/ISBN: 0032-3756
PMID: 2267191
Document Number: 362745
Transfection technique with the use of high molecular DNA was applied for the investigations of peripheral blood white cells DNA transforming properties in patients with chronic lymphatic leukemia B. Using transfection tests in liquid medium (focus assay) and colony forming in soft agar, the differences in L(tk-) cells transformation were noted following an addition of neoplastic DNA. Medium collected over transformants has shown mitogen properties in micro-mitogen tests on cells BALB/c 3T3. No correlation between clinical stage of the disease and the results of tests was observed.

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