Rapid isolation of substrate-quality plasmid DNA without CsCl-dye density gradients
Aguilar-Cordova, E.; Fontes, J.
Biotechniques 9(5): 538-540
1990
ISSN/ISBN: 0736-6205 PMID: 2268418 Document Number: 360544
The isolation of plasmid DNA produced in transformed bacterial cells is essential for many molecular biology techniques. Two drawbacks to the widely used CsCl-ethidium bromide method of preparation are the need for ultracentrifuge time and the generation of ethidium bromide waste. In this article we describe a method for the quick isolation of plasmid DNA without the use of an ultracentrifuge or ethidium bromide.