Use of a nested primer pair as control for PCR amplification of ribosomal DNA internal transcribed spacers in fungi
Molina, F.I.; Geletka, L.M.; Jong, S.C.; Zhang, Y.
Biotechniques 16(6): 998
1994
ISSN/ISBN: 0736-6205 PMID: 7915527 Document Number: 3601
We have described a double-nested primer pair that can be used to confirm the identity of first-round amplicons generated with the ITS I and ITS4 primers. Our studies show that the 5.8S F and R primers amplify the correct segment of the 5.8S gene located between the two ITS in diverse fungal taxa. A second-round PCR with these primers should be useful in restriction analyses and phylogenetic studies of the 5.8S+ITS rDNA for rapidly checking the nature of the first-round amplicons. We have successfully employed these primers in molecular characterization studies of diverse fungal genera at ATCC.
Document emailed within 1 workday
