Phosphatidylinositol-attached 5'-nucleotidase from synaptic plasma membrane of rat brain
Lai, K.M.; Wong, P.C.
Biochemistry International 22(5): 867-876
1990
ISSN/ISBN: 0158-5231 PMID: 1965942 Document Number: 358756
Synaptic plasma membranes (SPM) of rat brain contained a 5'-nucleotidase that was specifically released by Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PIPLC). About 30% of the enzyme was readily released and the remainder was less susceptible. Purified 5'-nucleotidase was treated with PIPLC and the resultant enzyme was almost totally partitioned into the detergent-poor phase following phase-separation in Triton X-114 indicating that PIPLC converted the enzyme from an amphipathic to a hydrophilic form. The results suggest that 5'-nucleotidase is anchored into SPM by a covalently attached phosphatidylinositol moiety.