A two-dimensional acrylamide gel electrophoresis/computer software approach to decoding the human genome
Krauss, M.R.; Collins, P.J.; Blose, S.H.
Biotechniques 8(2): 218-223
1990
ISSN/ISBN: 0736-6205 PMID: 2317378 Document Number: 358673
The human genome sequencing initiative is sure to be an expensive and time-consuming project. To maximize the quality of the initial thrust, it is necessary to concentrate on sequencing those genes that are actively involved in crucial biological processes, both normal and otherwise. To identify the protein products of these genes, we propose the use of two-dimensional acrylamide gel electrophoresis and powerful computer software. The two-dimensional gels can then be scaled to yield sufficient amounts of the protein for direct amino acid sequence analysis. The amino acid sequence then provides the direct link back to the gene.