Idiopathic myelofibrosis serum induces increased DNA synthesis in BALB/c 3T3 cells

Polato, G.; Pietrogrande, F.; Caenazzo, A.; Piva, E.; Burlina, A.; Girolami, A.

Folia Haematologica 117(3): 469-473

1990


ISSN/ISBN: 0323-4347
PMID: 1703119
Document Number: 358511
The effect of heated serum at a concentration of 10% in culture on the in vitro growth of confluent Balb/c 3T3 cells was studied in nine patients with Idiopathic Myelofibrosis and ten normal subjects. Patients showed significant increase in the mitogenic activity in comparison with normals. The growth factors conceivably implied for the observed effect are discussed. Particular attention is paid to Platelet-Derived Growth Factor from which serum mitogenic activity is primarily derived and is thought to take part in the genesis of bone marrow fibrosis.

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