Zinc-dependent angiotensin-converting enzyme in reactions with various enzymes
Kost, O.A.; Sharafutdinov, T.Z.; Kazinskaia, N.F.
Biokhimiia 55(8): 1396-1401
1990
ISSN/ISBN: 0320-9725 PMID: 1963088 Document Number: 358417
The effects of Zn2+ on the activity of the angiotensin-converting enzyme from bovine lung towards the substrates, FA-Phe-Gly-Gly and Cbz-Phe-His-Leu, have been studied. At pH below 7.0 zinc ions added to the reaction mixture increase the enzyme activity; this stimulating effect is changed to inhibition with a further rise in Zn2+ concentration. It was shown that the dissociation constant for the enzyme--Zn2+ complex and the "optimal" concentrations of Zn2+ needed for the manifestation of the maximal enzymatic activity depend on the nature of the substrate at all pH values studied.