A study of hexokinase isoenzymes in rat sarcoma M-1
Garkusha, N.F.; Goncharova, N.I.
Biokhimiia 55(8): 1519-1526
1990
ISSN/ISBN: 0320-9725 PMID: 2288991 Document Number: 355145
Using a wide spectrum of criteria, the isozyme composition of hexokinase from sarcoma M-1 reinoculated to rat m. gastrocnemius was studied. The structural, physicochemical and functional properties of the homogeneous enzyme which is represented in sarcoma M-1 by one molecular form, were investigated. Some properties of the enzyme (amino acid composition, resistance to mild proteolysis, Mr, pH-dependence of enzyme activity, electrophoretic mobility, kinetic behaviour) indicate that sarcoma M-1 hexokinase is a specific form of the enzyme which differs markedly from other known isozymes of mammalian hexokinase. The observed peculiarities of sarcoma M-1 hexokinase are discussed in terms of present-day concepts on the structure of isozymic spectra of enzymes in neoplastic tissues.