Top Abstract Purchase Related
Home
>
Node
>
Subnode

Sickle discocytes form more rouleaux in vitro than normal erythrocytes

Obiefuna, P.C.; Photiades, D.P.

Journal of Tropical Medicine and Hygiene 93(3): 210-214

1990

ISSN/ISBN: 0022-5304
PMID: 2348499
Document Number: 352881
Rouleaux formation of washed erythrocytes was studied in 32 normal (HbAA) individuals and 30 sickle cell anaemia (HbSS) patients. Washed red cells were suspended in their plasma and rouleaux formation visualized with the aid of an inverted microscope connected to a video camera-monitor unit. Aggregation rate was studied by visual particle counting. Washed sickle discocytes showed rouleaux formation three times as high as normal cells. Red cells were also suspended in buffered Ringer solution containing dextran (a rouleaux inducer). Again, washed erythrocytes from sicklers were about 3 times more aggregable than normal cells at all pH values studied. Increased acidity appeared to aggravate rouleaux formation in both Hb genotypes. We also found plasma fibrinogen concentration in sicklers to be about twice as high as that of normals. High rouleaux formation in sicklers could be attributed not only to fibrinogen and other plasma factors but also to cellular factors.

Document emailed within 1 workday
Secure & encrypted payments

Jones, G.L. 1979: Spin label study of hemoglobin membrane interactions in normal and sickle erythrocytes Proceedings of the Western Pharmacology Society 22: 79-86
Maniatis, A.; Bertles, J.F. 1978: Erythrocyte I-i antigens: distribution in normal and sickle erythrocytes and relationship of i antigenicity to hemoglobin F cell content Progress in Clinical and Biological Research 20: 159-171
Cimminiello, C.; Bernasconi, P.; Chianese, R.; Uberti, T.; Pietra, A.; Gibelli, A. 1987: Ticlopidine administration to patients does not inhibit the ability of their monocytes to form rosettes and to phagocytize erythrocytes in vitro International Journal of Tissue Reactions 9(3): 251-253
Reinhart, W.H.; Zehnder, L.; Schulzki, T. 2009: Stored erythrocytes have less capacity than normal erythrocytes to support primary haemostasis Thrombosis and Haemostasis 101(4): 720-723
Berkowitz, L.R.; Orringer, E.P. 1985: Passive sodium and potassium movements in sickle erythrocytes American Journal of Physiology 249(3 Pt 1): C208-C214
Basu, M.K.; Lee, M.M.; Maniatis, A.; Bertles, J.F. 1984: Characteristics of I and i antigen receptors on the membrane of erythrocytes in sickle cell anemia Journal of Laboratory and Clinical Medicine 103(5): 712-719
Jain, N.C.; Kono, C.S. 1977: Fusiform erythrocytes in Angora goats resembling sickle cells: influence of temperature, pH, and oxygenation on cell shape American Journal of Veterinary Research 38(7): 983-990
Boĭtsova, L.I.; Potapova, T.V. 1974: Capacity to form intercellular contacts of normal mouse fibroblasts and L-cells cultivated in a mixed culture with normal fibroblasts Biulleten' Eksperimental'noi Biologii i Meditsiny 78(8): 85-87
Whiteley, M.S.; Fox, A.D.; Thompson, M.M. 1997: Career aspirations and expectations of vascular trainees in 1996. The Rouleaux Club Annals of the Royal College of Surgeons of England 79(5 Suppl): 195-199
Metelkin, A.N.; Manin, V.N.; Rumiantsev, A.G. 1980: Analysis of the form and size of erythrocytes in healthy children and those with hereditary spherocytosis by the method of holographic interferometry Pediatriia 5: 43-45
Birmingham, D.J.; Shen, X.P.; Hourcade, D.; Nickells, M.W.; Atkinson, J.P. 1994: Primary sequence of an alternatively spliced form of CR1. Candidate for the 75,000 M(r) complement receptor expressed on chimpanzee erythrocytes Journal of Immunology 153(2): 691-700
Haynes, J.; Seibert, A.; Shah, A.; Taylor, A. 1990: Normal versus sickle red blood cells: hemodynamic and permeability characteristics in reperfusion lung injury Journal of the Association for Academic Minority Physicians: the Official Publication of the Association for Academic Minority Physicians 1(3): 62-66
Soprunov, F.F.; Benkowitsch, J.I.; Kazarinskaja, T.I. 1977: Functional characteristics of normal and pathologic erythrocytes Acta Biologica et Medica Germanica 36(5-6): 783-791
Horiuchi, K.; Onyike, A.E.; Osterhout, M.L. 1996: Sickling in vitro of reticulocytes from patients with sickle cell disease at venous oxygen tension Experimental Hematology 24(1): 68-76
Pradhan, P.K.; Dua, H.K.; Kavishwar, W.K.; Agarwal, S. 1985: Frequency of glucose-6-phosphate dehydrogenase deficiency in individuals with normal and sickle cell haemoglobin--a preliminary study Indian Journal of Pathology and Microbiology 28(1): 61-65
Plagne, R.; Bidet, J.M.; Chollet, P.h.; bard, J.J.; Sauvezie, B. 1974: Influence of various parameters on the uptake of 86 Rb by normal human erythrocytes Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 168(2-3): 289-294
D'Eramo, N. 1973: On the basophilic granulations of erythrocytes in normal and pathological conditions Bollettino della Societa Italiana di Biologia Sperimentale 49(7): 422-425
Al-Jobore, A.; Minocherhomjee, A.M.; Villalobo, A.; Roufogalis, B.D. 1984: Active calcium transport in normal and abnormal human erythrocytes Progress in Clinical and Biological Research 159: 243-292
Cunningham, A.J.; Steele, E.J. 1981: Ontogeny of the autoimmune reaction in normal mice to antigens in erythrocytes and gut Clinical and Experimental Immunology 44(1): 38-48
McIntire, L.V.; Leverett, L.B.; Alfrey, C.P.; Lynch, E.C.; Zanger, B. 1980: Effect of transfusion on hemoglobin flow and oxygen delivery in patients with sickle cell anemia--in vitro evaluation Medical Research Engineering 13(2): 27-31