Characteristics of the products of thiamine degradation by molluskan thiaminase i
Puzach, S.S.; Gorbach, Z.V.
Voprosy Meditsinskoi Khimii 35(2): 82-84
1989
ISSN/ISBN: 0042-8809 PMID: 2741418 Document Number: 347436
Tiaminase I (EC 2.5.1.2) catalyzed degradation of thiamin in the reaction mixture free of substrate-acceptor. Thiamin exhibited properties of both substrate-donor and -acceptor of the group transferred by thiaminase I. Identification of the products developed after thiaminase reaction involving thiamin was performed using chromatography and radiometry. Kinetics of the reaction is discussed.