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Thyroglobulin gene expression in human thyroid cells in various types of thyroid pathology

Kadyrova, D.A.; Atakhanova, B.A.; Maksumova, U.P.; Turakulov, I.K.; Shipitsyna, G.I.

Molekuliarnaia Biologiia 23(4): 1101-1106

1989

ISSN/ISBN: 0026-8984
PMID: 2586502
Document Number: 346830
Highly purified thyroglobulin mRNA was isolated from human nodal euthyroid goiter. Full length cDNA was synthesized from 33S RNA by using reverse transcriptase in the presence of human placenta ribonuclease inhibitor. DNA complementary to human Tg mRNA was used in liquid hybridization experiments to determine the quantity of Tg mRNA. The amount of Tg mRNA in euthyroid nodal and congenital goiter was reduced. Tg specific mRNA was absent in thyroid cancer cells.

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