Research in similarities between EEP34 and lipocortin
Takahashi, H.; Oota, A.; Nakayama, R.; Kobayashi, R.; Sakuragi, S.
Nippon Ganka Gakkai Zasshi 93(1): 132-141
1989
ISSN/ISBN: 0029-0203 PMID: 2526571 Document Number: 345680
EDTA-extractable protein (EEP) is a major extrinsic protein of lens fiber membrane. The EEP of calf lens separated three major bands by SDS-PAGE, and the molecular weights of these major bands were 34 kilodalton (kd.), 33 kd. and 32 kd. Combination of EEP with F-actin and some kinds of phospholipid containing liposomes depends on Ca2+. In two-dimensional gel electrophoresis, some spots of the calf-EEP partially overlapped with spots of lipocortin, which is a substrate for epidermal growth factor receptor/kinase and an inhibitor of phospholipase A2. The 34 kd. protein of the EEP cross-reacted to the antibody prepared against lipocortin of pig's aorta: These results suggest the important function of EEP in lens.