Components of the human T lymphocyte antigen receptor complex, CD3-p28 and CD3-gamma, are biochemically distinct
Stewart, S.J.; Levy, R.
Journal of Laboratory and Clinical Medicine 114(4): 394-400
1989
ISSN/ISBN: 0022-2143 PMID: 2794751 Document Number: 345323
A recently described subunit of the T cell antigen receptor-CD3 complex, p28, is very similar to the mature CD3-gamma subunit with respect to electrophoretic mobility. We demonstrated, by one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and endo-beta-n-acetylglucosaminidase digestion, that CD3-p28 and CD3-gamma coexist in CD3-specific immunoprecipitates from the human T lymphocyte hybrid cell line 255.88, which was labeled by biosynthetic incorporation of radioactive amino acids. By partial proteolysis and peptide mapping, we demonstrated that CD3-p28 and CD3-gamma are distinct members of the CD3 complex.