A modified Brown and Hopps stain for identification of gram-positive and gram-negative microorganisms in glycol methacrylate-embedded tissues
Schwartz, D.A.; Munger, R.; Lazzaro, B.; Lumb, G.
Archives of Pathology and Laboratory Medicine 113(2): 181-183
1989
ISSN/ISBN: 0003-9985 PMID: 2464978 Document Number: 345314
Plastic embedding is a recently developed technique that has been shown to be superior to conventional paraffin embedding in the histopathologic identification of microorganisms in tissue specimens. This report describes a modification of the Brown and Hopps stain for the differentiation of gram-positive and gram-negative microorganisms in 3-micron-thick plastic sections. This technique is easily performed and has a rapid turnaround time. This staining method permits a more precise histopathologic diagnosis of infectious agents than is possible in paraffin-embedded tissues.