Zinc forms complexes with higher kinetical stability than calcium, 5-F-BAPTA as a good example
Csermely, P.; Sándor, P.; Radics, L.; Somogyi, J.
Biochemical and Biophysical Research Communications 165(2): 838-844
1989
ISSN/ISBN: 0006-291X PMID: 2512923 Document Number: 344602
Increasing interest is focused on the role of zinc in biological systems. A rapidly growing family of DNA-binding proteins contains "zinc-fingers", where zinc is bound to cysteine or histidine residues. On the other hand zinc is able to displace calcium from its binding sites and in this way it may modify calcium-mediated cellular processes. In the present report dissociation rates of Zn2+- and Ca2+-complexes with 5-F-BAPTA, a widely used NMR-active calcium indicator, have been measured by two-dimensional 19F NMR exchange spectroscopic methods. The resutls show that the lifetime of the Zn2+-complex is more than five times longer than that of the Ca2+-complex. The longer lifetime, when combined with a higher thermodynamical stability of the Zn2+-complex, may explain why, in some cellular processes, Zn2+ can compete with Ca2+ in spite of a presumably high [Ca2+]/[Zn2+] free ion concentration ratio.