An improved micro-incubation well for immunocytochemical staining procedures
Yu, S.M.; Tseng, T.M.
Journal of the Formosan Medical Association 88(5): 523-525
1989
ISSN/ISBN: 0371-7682 PMID: 2477494 Document Number: 342841
A shallow "micro-incubation well" was made with a piece of the square plastic slide and a glass slide. A circular hole (10-12 mm in diameter) was cut out of the center of the square plastic slide. This plastic slide was then attached to the glass slide with a drop of Epon-Araldite and cured in the 60 degrees C oven for 2 days. One to three small sections of tissue mounted within corresponding circles on an ordinary microscopic slide could be stained when placed upside-down on the well in a moisture chamber. The moisture chamber was modified by a disposable square Petri dish (100 x 15 mm) containing a small amount of water and a shallow slide stage which was made by cutting a half inch rim from the bottom of a round disposable Petri dish (100 x 15 mm). This method provided the following advantages in immunocytochemical staining: (1) an even distribution of micro-amounts of antibody solution for long periods of time, and (2) greatly reduced the cost of the immunostaining technique by using micro-amounts of antibody solution.