DNA polymorphism analysis of hereditary multiple exostoses in horses
Li, J.K.; Moloney, B.K.; Shupe, J.L.; Gardner, E.J.; Leone, N.C.; Elsner, Y.
American Journal of Veterinary Research 50(6): 978-983
1989
ISSN/ISBN: 0002-9645 PMID: 2569854 Document Number: 342604
Genomic DNA polymorphisms obtained by restriction fragment-length polymorphism from healthy horses and horses with hereditary multiple exostoses were analyzed. These DNA were digested by 12 restriction enzymes and were hybridized against 6 isotopically labeled oncogene probes. Hybridization was not detected with the viral oncogene, v-ras, which indicated this oncogene was absent in the equine genome. Oncogenes (c-raf-1, c-fes, c-myb, c-myc, and c-sis) were present and had similar hybridization patterns and signal intensities in DNA from healthy horses and horses with hereditary multiple exostoses. Unique and distinct restriction fragment-length polymorphisms were detected with the c-raf-1 probe only in BamHI- and PstI-digested equine DNA.