Simultaneous quantification of oestrogen and progesterone receptors by a ligand binding assay in frozen sections
Vollmer, G.; Helmchen, B.; Knuppen, R.
Journal of Clinical Chemistry and Clinical Biochemistry 27(12): 953-959
1989
ISSN/ISBN: 0340-076X PMID: 2614342 Document Number: 341525
We describe two modifications of a double-isotope assay for measuring the concentrations of oestrogen receptors and progesterone receptors in tumour cytosols and extrcts of frozen tumour sections and endometrial sections. The concentrations of these receptors are derived from single-point/isoelectric focussing assays after incubation of cytosols or section supernatants either with ([125I]vinyl)-nortestosterone and [3H]oestradiol or [125I]oestradiol and [3H]ORG 2058 (16.alpha.-ethyl-21-hydroxy-19-nor[6,73H]pregn-4-ene-3,20-dione). The concentrations of the oestrogen and progesterone receptors found in cytosols (r > 0.93) and extracts from sections (r > 0.8) by dual label assays are highly correlated with those found by single label assays. The method described represents an approch to the determination of oestrogen and progesterone receptors biochemically in an amount of tissue which is comparable to that needed for immunocytochemical procedures.