Morphological and immunohistochemical studies on degenerative changes of the retina and the optic nerve in neonatal rats injected with monosodium-L-glutamate
Azuma, N.; Kawamura, M.; Kohsaka, S.
Nippon Ganka Gakkai Zasshi 93(1): 72-79
1989
ISSN/ISBN: 0029-0203 PMID: 2750602 Document Number: 339055
Degenerative changes in the retina and the optic nerve were investigated morphologically and immunohistochemically following administration of monosodium-L-glutamate (MSG) in rats. MSG (5mg/g b.w.) was injected subcutaneously every other day for 5 times after birth. The retina and the optic disc was observed ophthalmoscopically at 1, 3 and 6 months after birth. At the same stage, morphological and immunohistochemical changes were also investigated under light microscopy. The neuron of the retinal ganglion cell was identified immunohistochemically with antiserum to neurofilament 200 kD (NF). Glial cells were stained with antisera to glial fibrillary acidic protein (GFAP) and S-100 beta protein, and myelin of oligodendrocytes was also stained with antiserum to myelin basic protein (MBP). Histologically the inner layer of the retina was selectively destroyed, and the optic nerve also showed degeneration, changing into a thin strand. The retinal vessels were narrow and coarse, however, they extended to the peripheral region. Although immunohistochemical staining with NF antiserum was scarcely detected both in the retina and in the optic nerve, glial stainings with GFAP and S-100 beta protein antisera were widely observed in the perivascular space of the retina and in the glial column of the optic nerve. These findings indicated that the ganglion cells and their neurons are significantly affected by MSG, but the retinal vessels and glial cells are rarely affected. Ophthalmoscopically, the optic discs of rats treated with MSG were small and deeply excavated. The vitreous vessels persisted in most cases even at 6th months after birth.