Determination of dextromethorphan and its major metabolite dextrorphan by gas chromatography
Kintz, P.; Mangin, P.; Lugnier, A.; Chaumont, A.J.
Annales de Biologie Clinique 47(4): 193-195
1989
ISSN/ISBN: 0003-3898 PMID: 2751152 Document Number: 334088
A gas chromatography assay has been developed for the determination of dextromethorphan and dextrorphan in human fluids. Drugs are extracted with chloroform-isopropanol-n-heptane (50/17/33, v/v) form alkalinized samples using cyclizine as an internal standard, analysed using a 3 p. cent OV-17 column, and detected by selective nitrogen detection. The lower detection limits are 0.4 and 0.7 micrograms/l for dextromethorphan and dextrorphan, respectively.