Methodologic variants of DNA blood stain analysis: extraction in agarose and digoxigenin marking
Prinz, M.; Berghaus, G.; Staak, M.
Beitrage Zur Gerichtlichen Medizin 47: 175-179
1989
ISSN/ISBN: 0067-5016 PMID: 2818476 Document Number: 334056
DNA was extracted from 1 microliter bloodstains on glass, cotton cloth and paper using proteinase K digestion in a solution containing low gelling temperature agarose. This method is advantageous for small amounts of source material because the purifying procedures of other methods that contain a risk of loss of DNA, are avoided. The recovered DNA was digested with Hae III and after Southern blotting, a Digoxigenin labeled probe (Amprobe, human Y-chromosome specific repeat) was successfully used for sex determination. With this non-radioactive method quantities of DNA smaller than 1.5 pg were detected.