Further simplification of the general analysis of the intercalative drug-DNA interaction
Maiti, S.; Bhattacharya, K.L.
Indian Journal of Biochemistry and Biophysics 26(2): 109-119
1989
ISSN/ISBN: 0301-1208 PMID: 2777318 Document Number: 333331
The application of McGhee and von Hippel's general equation [J. Mol. Biol., 86 (1974), 469-489, Eq. (15)] to the analysis of the interaction of intercalative drugs with DNA has been further simplified. The value of n can now be determined mathematically, using a simple function, and without any approximation. It is also established that the summation of squared deviation of (( (nu/c), nu)) points would be minimum for and only for the true set of (K,n,omega) of the interaction system. The method incorporating the simplification has been applied to determine the binding parameters of adriamycin-DNA interaction according to the above general equation.