Release of cyclooxygenase products from primary cultures of tracheal epithelia of dog and human
Widdicombe, J.H.; Ueki, I.F.; Emery, D.; Margolskee, D.; Yergey, J.; Nadel, J.A.
American Journal of Physiology 257(6 Pt 1): L361-L365
1989
ISSN/ISBN: 0002-9513 PMID: 2514604 Document Number: 332672
Release of cyclooxygenase products from primary cultures of dog or human tracheal epithelium was measured by radioimmunoassay. In both species, bradykinin, platelet-activating factor (PAF), and A23187 (a calcium ionophore) caused increases in the rate of release of prostaglandin (PG) E2 and smaller increases in PGF2.alpha., 6-keto-PGF1.alpha., and thromboxane B2 output. Isoproterenol, vasoactive intestinal peptide (VIP), methacholine, and leukotrienes C4 and D4 had no effect on release of these cyclooxygenase products. Gas chromatography-mass spectrometry showed that the ratio of PGE2 to PGD2 released from dog cells by A23187 was 30:1. Short-circuit current across dog cells was stimulated by bradykinin, A23187, PAF, VIP, methacholine and isoproterenol. Only the responses to bradykinin and A23187 were reduced by pretreatment with indomethacin.