Isolation of macrophage-activating phospholipid from Listeria monocytogenes
Jakoniuk, P.; Sacha, P.; Borowski, J.
Medycyna Doswiadczalna i Mikrobiologia 41(1): 15-21
1989
ISSN/ISBN: 0025-8601 PMID: 2503663 Document Number: 331535
The aim of this study was isolate a factor, contained in phospholipid fraction of Listeria monocytogenes, responsible for macrophage activation. Lipids extracted from L. monocytogenes cells were subjected consecutively to fractionation on colons with silicic acid, Florisil and Sephadex LH-20. Separated fractions were assayed for their ability to activate macrophages as well as for their homogeneity by thin layer chromatography. Multistep fractionation procedure allowed to obtain the pure preparation of glycerophospholipid with a high ability for macrophage activation and capable to increase significantly anti-infections immunity in mice.