Kinetics of dissolution of solid protein substrates by proteinases. Selection of the reaction mechanism
Eremeev, N.L.; Kariakin, A.A.; Kazanskaia, N.F.
Biokhimiia 54(3): 503-510
1989
ISSN/ISBN: 0320-9725 PMID: 2665827 Document Number: 330978
Experimental data on the rate of enzymatic degradation of a solid protein phase in relation to reagent concentrations in the gelatine gel - proteolytic enzyme solution system was obtained. It was shown that different transformations of the Michealis-Menten equation do not provide any satisfactory description of the reaction kinetics. A method for the discrimination of putative reaction mechanisms based on the analysis of effective values of constants in the general equation for the rates of dissolution of proteins with different molecular weight was proposed. The experimental results are in favour of a two-step consecutive mechanism taking acount of surface concentrations of the adsorbed enzyme and hydrolyzeable bonds.