Optimizing electroporation parameters for a variety of human hematopoietic cell lines
McNally, M.A.; Lebkowski, J.S.; Okarma, T.B.; Lerch, L.B.
Biotechniques 6(9): 882-886
1988
ISSN/ISBN: 0736-6205 PMID: 3273199 Document Number: 326303
The parameters affecting electroporation of four human hematopoietic cell lines were investigated. The optimal conditions for electroporation are described for both transient and stable expression of foreign genes. A correlation exists between the levels of transient gene expression and stable transfection frequency. In addition, linear DNA yields higher stable transfection frequencies than supercoiled DNA. The cumulative results indicate that electroporation is a simple and useful method for obtaining transient and stable expression of foreign genes in human hematopoietic cells.