The use of nonradioactive DNA probes for the characterization of Leishmania isolates from Peru
López, M.; Montoya, Y.; Arana, M.; Cruzalegui, F.; Braga, J.; Llanos-Cuentas, A.; Romero, G.; Arévalo, J.
American Journal of Tropical Medicine and Hygiene 38(2): 308-314
1988
ISSN/ISBN: 0002-9637 PMID: 3354765 Document Number: 325019
We describe conditions for dot blot DNA hybridization studies using biotinylated kDNA probes from Leishmania. The sensitivity and specificity attained with biotinylated or 32P-labelled probes were equivalent. The lower level of detection obtained was 100 parasites that were blotted on nitrocellulose paper and then treated with Proteinase K. Studies were performed with 112 Leishmania isolates from Andean (uta) and sylvatic mucocutaneous (espundia) patients and all were determined to belong to the Leishmania braziliensis complex.
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