A novel blood group A- and O (H) -antigens--glycosphingolipids from porcine erythrocytes
Sako, F.
Hokkaido Journal of Medical Science 63(4): 534-544
1988
ISSN/ISBN: 0367-6102 PMID: 3220436 Document Number: 324051
Five neutral glycosphingolipids (GSLs) (Gb4Cer, Gb3Cer, LacCer, GlcCer and GalCer) were isolated from porcine erythrocyte membranes. Gb4Cer was the most abundant (over 70% of total neutral GSLs). In addition, a minor GSL fraction (less than 1% of the total), which migrated more slowly than Gb5Cer on TLC, composed of five GSLs. Two of them exhibited blood group A and O (H) activity, respectively, and were studied for their structures by spectrometries with NMR and FAB-mass, sequential hydrolysis by exoglycosidases, methylation and immunostaining. Chemical structure of the blood group A-antigen was proposed to be GalNAc alpha 1-3 (Fuc alpha 1-2) Gal beta 1-3 Gal-4Glc beta 1NAc beta 1-3 Gal beta 1-4 Glc beta 1-1Cer and GalNAc alpha 1-3 (Fuc alpha 1-2) Gal beta 1-4 GlcNAc beta 1-3 Gal beta 1-4Glc beta 1-1Cer. The blood group O (H)-antigen showed the structure lacking non-reducing terminal alpha-linked N-acetylgalactosamine from the A-antigen, or a biosynthetic precursor from of the A-antigen.