Endothelial cell injury in human saphenous veins after manipulation and tweezer grasping
Dregelid, E.; Svendsen, E.
Journal of Cardiovascular Surgery 29(4): 464-469
1988
ISSN/ISBN: 0021-9509 PMID: 3417749 Document Number: 321864
Eight pieces of human saphenous vein were subjected to tweezer grasping and digital handling. The pressure between the tweezer branches was estimated to be 2 .times. 105 Pa. The resulting endothelial injury was studied by light microscopy (LM), scanning (SEM) and transmission electron microscopy (TEM). Tweezer-grasping resulted in focal destruction of the endothelial lining, whereas digital handling (rolling the vein 3-4 times between the gloved fingers) resulted in minor morphological endothelial cell alterations.