A common disinfectant used in condom processing inhibits endonuclease digestion of sperm DNA
Fadda, S.; Pelotti, S.; Pappalardo, G.
International Journal of Legal Medicine 104(5): 281-284
1991
ISSN/ISBN: 0937-9827 PMID: 1723622 DOI: 10.1007/bf01369586Document Number: 321515
DNA recovered from a condom found at a crime scene appeared undigestible with restriction enzymes, preventing characterization by Southern blot and polymorphic probe hybridization. Several chemical substances used in the processing and treatment of condoms were tested for inhibitory action of restriction enzymes. In particular dibenzalkonium chloride appeared to promote enzyme inhibition at very low concentrations. The effectiveness of treatments to restore cleavage of sample DNA in the presence of such contaminants is discussed.
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