Fecal culture of Mycobacterium paratuberculosis in cattle using a centrifugation specimen processing step
Kim, Y.G.; Bech-Nielsen, S.; Gordon, J.C.; Slemons, R.D.; Spangler, E.
Acta Veterinaria Scandinavica. Supplementum 84: 203-205
1988
ISSN/ISBN: 0065-1699 PMID: 3232608 Document Number: 320480
One hundred and thirtyone faecal samples from cattle clinically suspected of having paratuberculosis were processed using the sedimentation culture method (SM) and the centrifugation culture method (CM) before inoculation into Herrold's egg yolk medium containing mycobactin J (M1), control medium as M1 with no mycobactin J (M2), medium as M1 plus miconazole (M3) and control medium for M3 which did not contain mycobactin J (M4). On M1 media the isolation rates were 40.4% for SM and 45.8% for CM, on M3 the isolation rates were 35.8% and 41.2%, respectively. The difference of 5.4% between the two methods was not statistically significant. Contamination rates of the sedimentation culture method were 25.9% on M1 and M2 media and 27.5% on M3 and M4 media, which were not statistically different. For the centrifugation method the rates were significantly increased (60.3% and 37.4%, respectively). The difference of 22.9% was statistically significant.