Application of HPLC with a metal-free column to the analysis of cyclosporin in whole blood samples from cardiac transplant patients
Loo, J.C.; Jordan, N.; McGilveray, I.J.
Research Communications in Chemical Pathology and Pharmacology 61(2): 261-264
1988
ISSN/ISBN: 0034-5164 PMID: 3055108 Document Number: 318210
A simplified, reverse-phase HPLC method utilizing a metal-free column was developed for the analysis of cyclosporin (CYA) in whole blood in humans. The average intra and inter assay variation at the low end of the therapeutic range are less than or equal to 4.5 and 8.8% respectively. The procedure was successfully applied to the measuring of CYA in whole blood collected from cardiac transplant patients over a one year period.
Document emailed within 1 workday
Related Documents
Lomeo, A.; Bolner, A.; Scattolo, N.; Guzzo, P.; Amadori, F.; Sartori, S.; Lomeo, L. 2008: HPLC Analysis of HbA1c in Dried Blood Spot Samples (DBS): a Reliable Future for Diabetes Monitoring Clinical Laboratory 54(5-6): 161-167Lipkin, G.W.; Tucker, B.; Giles, M.; Raine, A.E. 1993: Ambulatory blood pressure and left ventricular mass in cyclosporin- and non-cyclosporin-treated renal transplant recipients Journal of Hypertension 11(4): 439-442
Weller, S.; Thürmann, P.; Rietbrock, N.; Gossmann, J.; Scheuermann, E.H. 1995: HPLC analysis of azathioprine metabolites in red blood cells, plasma and urine in renal transplant recipients International Journal of Clinical Pharmacology and Therapeutics 33(12): 639-645
Matharu, G.S.; Berryman, F.; Brash, L.; Pynsent, P.B.; Treacy, R.B.; Dunlop, D.J. 2018: The effectiveness of blood metal ions in identifying bilateral metal-on-metal total hip arthroplasty patients at risk of adverse reactions to metal debris Acta Orthopaedica Belgica 84(2): 154-162
Kokot, F.; Cekański, A.; Kuska, J. 1974: Concentration of glucose, insulin and free fatty acids in arterial blood samples of pregnant women and cord blood samples of their fetuses Ginekologia Polska 45(2): 157-165
Sanguansermsri, T.; Thanaratanakorn, P.; Steger, H.F.; Tongsong, T.; Sirivatanapa, P.; Wanapirak, C.; Sirichotiyakul, S.; Chanprapas, P.; Flatz, G. 2001: Prenatal diagnosis of hemoglobin Bart's hydrops fetalis by HPLC analysis of hemoglobin in fetal blood samples Southeast Asian Journal of Tropical Medicine and Public Health 32(1): 180-185
Larsson, M.; Forsman, A.; Hallgren, J. 1987: HPLC assays of noradrenaline and dopamine in CSF samples from drug-free subjects Methods and Findings in Experimental and Clinical Pharmacology 9(10): 669-672
Veremis, S.A.; Maddux, M.S.; Tang, I.Y.; Millner, S.N.; Prasad, R.; Pollak, R.; Mozes, M.F. 1989: Comparison of cyclosporine (CSA) blood levels and RIA/HPLC ratios among renal transplant patients with normal allograft function, CSA nephrotoxicity or rejection Transplantation Proceedings 21(1 Part 2): 1476-1477
Filingeri, V.; Baroni, B.; Boffo, V.; Rosati, R.; Pisani, F.; Nardi, S.; Casciani, C.U. 1987: Effects of cyclosporin on blood gastrin after kidney transplant Minerva Medica 78(12): 831-834
Shinpo, K.; Kawai, K.; Shinzato, M.; Ito, S.; Fujita, K.; Matsui, T.; Nakamura, K.; Kitagawa, Y.; Hirano, M.; Ito, M. 1984: Urinary polyamine analysis and its clinical significance. I. Analysis by HPLC and its clinical application. A. Urinary acetylpolyamine in patients with malignant tumors Rinsho Byori. Japanese Journal of Clinical Pathology Spec no 59: 29-37
Eguchi, K.; Yonezawa, M.; Ohmoto, H.; Hayase, R.; Sekiba, K. 1984: Urinary polyamine analysis and its clinical significance. I. Analysis by HPLC and its clinical application. B. Clinical application to the fields of obstetrics and gynecology Rinsho Byori. Japanese Journal of Clinical Pathology Spec no 59: 38-49
Weissgarten, J.; Ng, C.; Freidman, E.; Cardella, C.J. 1989: B lymphocytes from stable renal transplant patients do not respond to exogenous lymphokines: comparison of azathioprine and cyclosporin treated patients Transplantation Proceedings 21(1 Part 1): 393-394
Pisani, F.; Meloni, C.; Morosetti, M.; Tozzo, C.; Meschini, L.; Mazzarella, V.; Iacona, A.; Manca Di Villahermosa, S.; Taccone-Gallucci, M.; Casciani, C.U. 1988: Adverse effects of the use of cyclosporin a in patients having undergone a kidney transplant Minerva Urologica e Nefrologica 40(1): 73-76
Shaikh, A.; Arendorf, T.M. 1991: Cyclosporin-induced gingival overgrowth in renal transplant patients South African Medical Journal 80(1): 61-62
Wilmink, J.M.; Bras, J.; Surachno, S.; van Heyst, J.L.; van der Horst, J.M. 1989: Bone repair in cyclosporin treated renal transplant patients Transplantation Proceedings 21(1 Part 2): 1492-1494
Griffith, B.P.; Hardesty, R.L.; Trento, A.; Lee, A.; Bahnson, H.T. 1984: Targeted blood levels of cyclosporin for cardiac transplantation Journal of Thoracic and Cardiovascular Surgery 88(6): 952-957
Ohnuma, O.; Shirata, T.; Suzuki, T.; Sato, M.; Kanouchi, K.; Noguchi, N.; Shiraiwa, T.; Kikuchi, M.; Sato, M.; Seino, F.; Okuyama, Y.; Igarashi, M. 2010: Improvement of the system for collecting blood samples, at the Division of Clinical Laboratory in Yamagata University Hospital, based on the complaints of patients: The way to perform the newly-developed 30-min changeover method for collecting blood samples by medical technologists Rinsho Byori. Japanese Journal of Clinical Pathology 58(6): 581-585
Costa, C.; Sidoti, F.; Mantovani, S.; Gregori, G.; Proietti, A.; Ghisetti, V.; Cavallo, R. 2016: Comparison of two molecular assays for detection of cytomegalovirus DNA in whole blood and plasma samples from transplant recipients New Microbiologica 39(3): 186-191
Izotova, N.P.; Grudtsyn, G.V.; Gratsianskiĭ, N.A. 1973: Content of free fatty acids in the blood of patients with acute myocardial infarct and disorders of the cardiac rhythm Kardiologiia 13(11): 79-83
Matl, I.; Gintelová, J.; Jirka, J.; Rossmann, P. 1993: Graft rejection after changing from cyclosporin A therapy to conventional treatment in kidney transplant patients Casopis Lekaru Ceskych 132(10): 301-304