Pueraria mirifica, phytoestrogen-induced change in synaptophysin expression via estrogen receptor in rat hippocampal neuron
Chindewa, R.; Lapanantasin, S.; Sanvarinda, Y.; Chongthammakun, S.
Journal of the Medical Association of Thailand 91(2): 208-214
2008
ISSN/ISBN: 0125-2208 PMID: 18389986 Document Number: 3052
Objective: To examine Pueraria mirifica (Leguminosae) containing-phytoestrogen effect on synaptic density and involvement of estrogen receptor.Material and Method. The level of synaptophysin, a presynaptic vesicle protein, was measured using Western blot analysis and immunocytochemistry in hippocampal primary cell cultures at 6 days in vitro.Results: P. mirfica and 17 beta-estradiol (0.1 mu M) treatment for 4 days, but not for 2 days, significantly increased synaptophysin immunoreactivity and level of synaptophysin. R mirifica up to 60 mu g/ml resulted in a dose related increase in the level of synaptophysin immunoreactivity. The classical estrogen receptor antagonist, ICI 182 780, significantly blocked P. mirifica-induced increase in synaptophysin.Conclusion: P. mirifica-containing phytoestrogen affects synaptic density by inducing synaptophysin expression via estrogen receptor.
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