Regulation of SV40 DNA replication by phosphorylation of T antigen
Mohr, I.J.; Stillman, B.; Gluzman, Y.
EMBO Journal 6(1): 153-160
1987
ISSN/ISBN: 0261-4189 PMID: 3034573 Document Number: 303867
The role of phosphorylation in regulating the biochemical properties of SV40 large T antigen has been examined. Treatment of purified T antigen with calf intestinal alkaline phosphatase resulted in the removal of 80% of the 32P label. This partially dephosphorylated T antigen displayed an increase in its ability to support DNA replication in vitro. This increase in replication activity was paralleled by an activation of specific DNA binding to site II, a necessary element within the origin of SV40 DNA replication. In contrast, the ATPase activity of dephosphorylated T antigen remained unchanged. These results demonstrate that DNA replication is regulated by phosphorylation of an origin specific DNA binding protein.