Extra reactivities detected in flow-cytometry-positive, CDC-negative crossmatches are definable HLA specificities
Rodey, G.; Bollig, B.; Oldfather, J.; Schutte, L.; Flye, W.; Phelan, D.
Transplantation Proceedings 19(1 Part 1): 778-779
1987
ISSN/ISBN: 0041-1345 PMID: 3274872 Document Number: 301122
In this preliminary study, additional reactions were detected in sera that were not found by T-AHG-CDC. The reactions had definable HLA specificities. In our laboratory, the procedures described in this article had the following relative sensitivities for detecting class I HLA alloantibody specificities: FC = B-AHG-CDC greater than T-AHG-CDC greater than B-CDC greater than T-CDC. This study supports the concept that some FC-positive crossmatches, negative by T-AHG-CDC, can be associated with reduced renal allograft survival, since many of the additional reactions detected by FC appear to be due to HLA Class I antibodies.