The degree of modification of serum albumin as a toxicological test

Troitskiĭ, G.V.; Kasymova, G.A.; Borisenko, S.N.

Voprosy Meditsinskoi Khimii 33(2): 38-41

1987


ISSN/ISBN: 0042-8809
PMID: 3604137
Document Number: 300318
A procedure is developed, involving electrophoresis and TCA-ethanol treatment of electrophoregramms, which enabled to estimate quantitatively the modified derivatives of albumin just in blood serum without the protein isolation. The method is based on ability of native albumin to maintain its solubility after TCA-ethanol treatment. At the same time, modified albumin with conformational alterations was fixed on a carrier and might be estimated using colorimetry or densitometry. The procedure developed might be used in chemical industry for evaluation of toxic effects of some substances.

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