Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast
Stern, P.L.; Beresford, N.; Friedman, C.I.; Stevens, V.C.; Risk, J.M.; Johnson, P.M.
Journal of Immunology 138(4): 1088-1091
1987
ISSN/ISBN: 0022-1767 PMID: 3543121 Document Number: 296501
Human placental villous trophoblast is known to be unreactive with W6/32 and other monoclonal antibodies recognizing monomorphic determinants of human class I MHC heavy chains, whereas extravillous cytotrophoblast in the placental bed is W6/32-reactive by immunohistology. We have now demonstrated in contrast, that syncytiotrophoblast is the only cellular component of baboon early placental villous tissue which is reactive with any of these antibodies. Radioimmunoprecipitation of detergent-solubilized baboon placental membrane preparations, and subsequent SDS-PAGE, has shown the W6/32-reactive component to have an m.w. of 41,000 and to be associated with .beta.2-microglobulin, whereas baboon peripheral lymphocytes express 45,000 m.w. W6/32-reactive antigens comparable with the HLA-A,B,C heavy chains of human lymphocytes.