Monoclonal antibody to human lung angiotensin-converting enzyme
Danilov, S.M.; Allikmets EYu; Sakharov IYu; Dukhanina, E.A.; Trakht, I.N.
Biotechnology and Applied Biochemistry 9(4): 319-322
1987
ISSN/ISBN: 0885-4513 PMID: 2822061 Document Number: 294718
The hybridoma producing monoclonal antibody (IgG1) to human angiotensin-converting enzyme (ACE) has been prepared by fusion of murine myeloma P3O1 with spleen cells of BALB/c mice immunized with a purified human lung ACE preparation. A high specificity of monoclonal antibody (MAb) binding to immobilized ACE has been demonstrated by enzyme-linked immunosorbent assay and that of soluble ACE by an immunoadsorption test. The latter technique permits the use of impure ACE preparations for the screening procedure. This MAb did not affect ACE activity. We believe this antibody will be useful not only for immunoassay and immunopurification of ACE, but also as a tool for the investigation of the tissue distribution of the enzyme as well as for the study of the structure and mechanism of action of ACE.