Subcellular distribution of glucocorticoid receptor in the neoplastic epithelial duct cell line from human salivary gland
Kurokawa, R.; Kyakumoto, S.; Hatakeyama, S.; Ota, M.
Biochemistry International 14(1): 37-44
1987
ISSN/ISBN: 0158-5231 PMID: 3566776 Document Number: 293464
Neoplastic epithelial duct cell line from human salivary gland (HSG cell line) contains the specific glucocorticoid receptor. The time course study on the uptake of [3H]triamcinolone acetonide (TA), a synthetic glucocorticoid, by intact HSG cells in a growing monolayer culture showed that translocation of glucocorticoid receptors into nuclei occurred at 37.degree. C, but not at 0.degree. C. To elucidate the subcellular distribution of glucocorticoid receptor from HSG cells, a scaled-up-culture was employed. When the cells were incubated with [3H]TA at 0.degree. C, 94% of the receptors were found in the cytosol fraction, while only 6% of the receptors existed in the nuclei. When the cells were incubated at 37.degree. C, 49% of the receptor complexes were distributed in the nuclei and 74% of these nuclear receptor complexes were extractable with 5 mM pyridoxal phosphate.