Glyoxalase i typing and phosphoglucomutase-1 subtyping of a single hair
Gambel, A.M.; Budowle, B.; Terrell, L.
Journal of Forensic Sciences 32(5): 1175-1181
1987
ISSN/ISBN: 0022-1198 PMID: 2959745 Document Number: 293110
A technique is described for the typing of glyoxalase I (GLO I) and the subtyping of phosphoglucomutase-1 (PGM-1) from the root sheath cells of a single forcibly removed hair. This procedure does not require sample preparation and does not alter the morphological characteristics of the hair. The combined discrimination probability (DP) of the two markers taken together is 0.90 for whites and 0.89 for blacks. GLO I can be typed after four weeks, and PGM-1 can be typed after eight to fifteen weeks in hairs maintained at room temperature. Hairs mounted with Permount showed loss of enzyme activity and loss of band sharpness.