Factors of tissue-specific transcription of immunoglobulin kappa genes
Stepchenko, A.G.; Luchina, N.N.; Adzhalov, V.A.; Polianovskiĭ, O.L.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 8: 14-16
1987
ISSN/ISBN: 0208-0613 PMID: 3119996 Document Number: 292021
Proteins TIF1 and TIF2 with mol mass 90 and 94 kD, respectively, were found in myeloma cells. They bind to the promoter region of the kappa genes. Protein TIF1 was found in nonlymphoid cells (HeLa, liver cells). From myeloma MPOC21 a fraction was isolated enriched 400 fold with TIF2 by fractionation on ion-exchangers DE52 and P11 and hydroxylapatite. The purified protein protected the DNA site that contained 30bp including the conservative sequence TNATTTGCAT located upstream of the kappa gene promoter against the action of DNAase. Addition of the purified factor to the extract from HeLa cells increased several fold the efficiency of transcription and corrected the initiation of transcription.