Induction of mouse monocyte-macrophage replication by a mesangial cell-derived factor
Ooi, B.S.; MacCarthy, E.P.; Weiss, M.A.; Hsu, A.; Ooi, Y.M.
Journal of Laboratory and Clinical Medicine 110(4): 466-474
1987
ISSN/ISBN: 0022-2143 PMID: 3309103 Document Number: 291363
Conditioned media of cultured mouse mesangial cells (possessing microfilaments) were shown to contain a factor that stimulated splenic monocytes-macrophages and blood monocytes to replicate. Replicated cells were shown to express MAC-1 antigen as demonstrated by immunofluorescence with anti-MAC 1 and to possess Fc receptors as evidenced by their capacity to ingest sensitized erythrocytes. Preliminary characterization revealed the following characteristics: by Amicon ultrafiltration, fractions greater than 100,000 daltons were shown to have biologic activity; chromatofocusing of these active fractions revealed a peak of activity associated with fractions having pH 4; heating to 100 degrees C for 10 minutes abolished almost all activity, whereas trypsin treatment was without effect. The observations suggest a mechanism by which mesangial cells may modulate the proliferation of monocytes-macrophages that infiltrate the glomerulus in glomerulonephritis.